The DetectX® Prolactin ELISA Kit is designed to quantitatively measure Prolactin present in biological samples and tissue culture media

检测类型: Sandwich ELISA
样本类型: Serum, Plasma, Tissue Culture Media
灵敏度: 11.7 pg/mL
适用物种: 人, Elephant, Seal
检测时间: 2.5小时
样品数/板: 40 in Duplicate
读数: Colorimetric, 450 nm

检测原理:
The Prolactin (PRL) ELISA Kit quantitatively measures Prolactin levels in serum, plasma, and tissue culture media. The Prolactin (PRL) ELISA Kit is a sandwich ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

Use our provided Prolactin standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our mouse anti-Prolactin antibody and incubate shaking at room temperature for 1 hour. Add the peroxidase-conjugated human Prolactin polyclonal antibody. Then incubate the mixture covered, shaking at room temperature for 1 hour. The immunological reaction occurs between the peroxidase-conjugated Prolactin polyclonal antibody and the Prolactin antigen in the sample or standard.

After the 1-hour incubation, wash away the excess peroxidase-conjugated Prolactin antibody and add the TMB substrate. The TMB substrate reacts with the bound peroxidase-conjugated Prolactin antibody generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Prolactin concentration in the samples.

背景:
Prolactin (PRL) is a polypeptide hormone synthesized and secreted from specialized cells of the anterior pituitary gland. This hormone was named Prolactin because an extract of the bovine pituitary gland would cause the crop sac’s growth and stimulate milk production in pigeons or promote lactation in rabbits. However, Prolactin has over 300 different biological activities. Prolactin has multiple roles in reproduction besides lactation and plays multiple homeostatic functions in the organism. Furthermore, numerous other organs and tissues besides the anterior pituitary can synthesize and secrete Prolactin.