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Block Ace powder:Blocking reagent 封闭剂/阻断剂
货号:UK-B80 | 规格:4g x20 | 价格:¥3250.00 | 品牌:KAC
Block Ace:Blocking reagent阻断剂适用于在进行酶联免疫吸附试验(ELISA)、放射免疫测定(RIA)和免疫印迹法时阻断抗原或抗体的非特异性结合。

Block Ace powder以乳蛋白为原料制备而成,是一种新型的免疫实验阻断剂。 由于乳蛋白中包含许多疏水基团,因此它对塑料和膜过滤器具有很高的亲和力。

因此,与传统的阻断剂如牛血清白蛋白(BSA)相比,Block Ace阻断剂适用于在进行酶联免疫吸附试验(ELISA)、放射免疫测定(RIA)和免疫印迹法时阻断抗原或抗体的非特异性结合。


关键特性

由于该产品具有高阻断作用,并将本底维持在低水平,因此在低浓度的检测中很有用,如ELISA。


经过热处理,牛源抗体、酶和病毒被灭活;

● 在加热和冷冻时是稳定的;

● 能稳定生理活性物质,可作为稀释剂使用;

● 与BSA溶液相比,Block Ace是经济的;

● 能在水中迅速溶解。


如何使用?

Four (4) grams of Block Ace powder is dissolved into 100 mL of purified water, and this solution is used as a concentrate.


Blocking

For blocking purpose, either concentrate or 2-4× diluted solution with purified water is used (4× diluted solution is used for ELISA; concentrate is used for Western Blotting).

Dilution

For dilution of samples and labelled antibody, it is diluted to 10× with purified water.

Washing

For washing operation such as B/F separation, it is diluted to 10× with purified water, and the required volume of Tween 20 (0.05%–0.2%) is added.


存储方法

Room temperature (it is cold transported)

(This product is heat treated, but there is no preservative or antibiotic. Once dissolved, it should be frozen before storage)


Block Ace与1%BSA溶液之间的封闭效果比较

Anti-human kappa chain antibody and anti-human ramda chain are coated, and it is blocked with either Block Ace or 1% BSA. Human IgG ELISA demonstrates that Block Ace has low background and a steep standard curve.



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