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Deoxyribonuclease I(DNase I) 脱氧核糖核酸酶I
E4640 BMASSAY
460.00 / 15ku
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Deoxyribonuclease I,即DNase I,中文名称:脱氧核糖核酸酶I,来源于牛胰腺(bovine pancreas)。

别名:脱氧核糖核酸5′-寡核苷酸-水解酶;Deoxyribonucleate 5′-oligonucleotido-hydrolase。

分子量 ~31 kDa,CAS号 9003-98-9,EINECS编号 232-667-0,EC Number 3.1.21.1,MDL编号 MFCD00130918。

级别:Molecular Biology Grade

Deoxyribonuclease I from bovine pancreas;
Type: Type IV;
Activity…………≥2,000 Kunitz units/mg protein;
Chymotrypsin………………………≤0.5%;
Protease…………………………………≤0.05%;
RNase…………………………………..≤0.02%;
solubility………0.15 M NaCl: soluble 5.0 mg/mL。

脱氧核糖核酸酶I(Deoxyribonuclease I)为类白色冷冻干燥粉末。溶于水,微溶于30%乙醇和丙酮。用于从蛋白质样品中除去DNA。

DNAse I is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines. It has also been used during RNA extraction from Sindbis virus.

Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas.

储存:冷冻(-20℃)。

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