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DEPC 焦碳酸二乙酯(Sigma)
D5758 BMASSAY
2760.00 / 100ml
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产品说明 / Product Overview:


DEPC即Diethyl pyrocarbonate,焦碳酸二乙酯.

别名:氧二甲酸二乙酯;重碳酸二乙酯;RNA酶清除剂;DEP;Diethyl dicarbonate;Pyrocarbonic acid diethyl ester;Oxydiformic acid diethylester。

分子式:C6H10O5, 分子量:162.14, CAS号 1609-47-8, MDL编号 MFCD00009106, EINECS编号216-542-8.

本品为Sigma-Aldrich原装产品,货号D5758-100ML。

性状:无色透明至微琥珀色液体,溶于乙醇、乙醚、丙酮,缓慢溶于水而水解。易挥发,用后请旋紧瓶盖。

Purity ………… ≥97% (NMR);
Density ………… 1.101 g/mL at 25 °C(lit.);
Refractive index ………… n20/D 1.398(lit.);
bp ………… 93-94 °C/18 mmHg(lit.).

Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution.

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases.It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.

在溶液中浓度约为0.1% (v/v) 时可使RNase失活,从而防止RNA被降解。

储存:2-8°C避光保存。

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  • DEPC焦碳酸二乙酯
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