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PolySAX LP™色谱柱
PolySAX PolyLC
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产品说明 / Product Overview:



PolySAX LP™ is a silica-based material with an adsorbed, cross-linked coating. It is a strong anionexchange(SAX) material. The capacity is high for both small and large solutes. Columns are shipped filled with
methanol. Flush new columns with at least 15 column volumes of water (30 ml for a 200 x 4.6-mm) then condition with a salt solution prior to initial use. The hydrophilic coating imbibes a layer of water of hydration and swells, especially in the presence of salt solutions. This causes a modest, irreversible increase in the column back pressure. Since the swelling increases the surface area of the coating, this increases the binding capacity for proteins as well. This process should be hastened by eluting the column with a concentrated salt solution for two hours prior to its initial use. A convenient solution for the purpose is 0.2 M NaH2PO4 + 0.3 M sodium acetate. Alternatively, if the column is to be used for proteins or peptides that may interact with heavy metals (e.g., phosphopeptides), then the column should be passivated by elution for 20-24 hours with 40 mM EDTA.2Na at a low flow rate.

New HPLC columns sometimes absorb small quantities of proteins or phosphorylated peptides in a nonspecific manner. The sintered metal frits have been implicated in this. Eluting the column for 20-24 hr. at a low flow rate with 40mM EDTA. 2Na usually solves the problem. This passivates all metal surfaces in the HPLC system, as well as the column[CAUTION: This treatment can affect the integrity of the frits in some cases, and should probably be avoided with columns packed with 3-µm material. In some cases this has also caused the collapse of 5-µm, 200-Å column packings].

Routine use: Columns should be used at ambient temperatures. Filter mobile phases and samples before use. Failure to do so may cause the inlet frit to plug. If a salt gradient is being used, flush the column with 15 ml of the high-salt buffer before equilibrating with the low-salt buffer. At the end of the day, flush the column with 15 column volumes of water and plug the ends.

Loading Capacity: The loading capacity of a 4.6mm ID column is about 4-5 mg of protein/injection, depending on the strength of the protein’s binding to the support.

Storage: 1) Overnight: 100% mobile phase A. 2) Several days: Store in water. 3) Longer periods: Store in water in the refrigerator, with the ends plugged. ACN can be added to the storage solvent (e.g., ACN:Water = 80:20) to retard microbial growth.

Column maintenance:
1) After several hundred operating hours, reverse the direction of flow and elute the column for several hours with a high-salt buffer. Continue using the column in the reverse direction.
2) Silica-based columns are slowly degraded at basic pH. Transient exposure to > pH 8.0 should have minimal effect on the column. If prolonged use above pH 8.0 is expected, then inserting a saturator column of inexpensive silica in-line before the injector valve will prolong column lifetime.

Anion-exchange of peptides: In contrast to proteins, acidic peptides can be run at lower pH down to the range where Asp- and Glu- residues start to lose charge (@ pH 4). Retention reaches a maximum around pH 4.5. Peak shape and selectivity can sometimes be improved by inclusion of 10% organic solvent (MeOH or ACN) in the mobile phase.

Anion-exchange of proteins: This is typically performed in the pH range 7.0-8.0, using either increasing salt or decreasing pH gradients. The higher the pH, the stronger the binding of proteins. Tris is a good buffer in this range. Since halide salts tend to corrode stainless steel, acetate salts are often used to generate the gradient. However, it may be necessary to use a chloride salt gradient if the absorbance is to be monitored below 235 nm. If so, flush with 15 column volumes of water at the end of the day to preclude corrosion. It is also helpful to passivate the column every 3-4 weeks with EDTA.2Na (@ above) if chloride salts are used with regularity.

• For proteins < 15 KDa, a 300-Å material can be used. For larger proteins, a 1000-Å column is recommended.

Anion-exchange of oligonucleotides and oligonucleotide analogs: For oligos < 25 bases long, use 300-Å material. For oligos > 25 bases long and dsDNA, use of a 1000-Å column will afford elution without excessive amounts of salt. It is helpful to include 25-50% acetonitrile or propanol in both mobile phases. Large oligos may require over 1 M salt for elution. It is advisable to use a chaotropic salt (e.g., NaBr or NaClO4) for the gradient in cases where there could be a tendency for self-association like GC-rich sequences; otherwise, ammonium acetate can be used.


PolySAX LP™订购信息:

色谱柱 --- 适用于3µm颗粒可选孔径:-01,-03,-10

柱尺寸

产品编号

50 x 1.0mm

051SX03--

100 x 1.0mm

101SX03--

50 x 2.1mm

052SX03--

100 x 2.1mm

102SX03--

35 x 4.6mm

3.54SX03--

50 x 4.6mm

054SX03--

100 x 4.6mm

104SX03--

100 x 9.4mm

109SX03--



色谱柱 --- 适用于5µm颗粒可选孔径:-03,-10

柱尺寸

产品编号

50 x 1.0mm

051SX05--

100 x 1.0mm

101SX05--

150 x 1.0mm

151SX05--

35 x 2.1mm

3.52SX05--

50 x 2.1mm

052SX05--

100 x 2.1mm

102SX05--

200 x 2.1mm

202SX05--

35 x 4.6mm

3.54SX05--

50 x 4.6mm

054SX05--

100 x 4.6mm

104SX05--

200 x 4.6mm

204SX05--

100 x 9.4mm

109SX05--

200 x 9.4mm

209SX05--

250 x 9.4mm

259SX05--

250 x 21.0mm

2521SX05--

 

毛细管--- 5µm颗粒(可选孔径:-01, -03, -10

50mm x 150µm

050.15SX05--

100mm x 150µm

100.15SX05--

150mm x 150µm

150.15SX05--

50mm x 300µm

050.30SX05--

100mm x 300µm

100.30SX05--

150mm x 300µm

150.30SX05--

3-µm毛细管5-µm价格一样(在孔径-01Å-10Å时)。产品编号:“05”改成“03”。

另有75µm100µm200µm内径可供选择,详情请咨询中昊新生科技

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