The wound healing assay has been widely adapted and modified to study the effects of a variety of experimental conditions, for instance, gene knockdown or chemical exposure, on mammalian cell migration and proliferation.
The ABS_Bio™ Wound Healing Assay Kit provides a simple, sensitive, one-step colorimetric and fluorimetric assay for cell migration and proliferation. In this assay, the inserts create a wound field with a defined gap of 0.5mm and the “healing” of this gap by cell migration and growth towards the center of the gap is monitored and quantitated. Factors that alter the motility and/or growth of the cells can lead to increased or decreased rate of “healing” of the gap. The Cell proliferation and migration rates can be determined using manual fixing and microscopic imaging. The kit is supplied with sufficient reagents for 25 tests in 12-well or 6-well plate assay. It could easily be modified for a high-throughput assay.