DetectX® Insulin Enzyme Immunoassay Kit
灵敏性 - 检测范围1600 - 100 pg/mL
适用样品 - 血清, 尿液和组织培养基
样品数/盒 - 42 复孔
标准 - 世界卫生组织 (WHO) 1975年第一届国际标准(66/304)
快速 - 检测时间仅需要2个小时
The DetectX Insulin EIA kit is designed to quantitatively measure insulin present in a variety samples and tissue culture media. An Insulin standard, calibrated to the WHO International Standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture insulin present in the sample. After a 60 minute incubation, the plate is washed. A peroxidase conjugated insulin antibody is added and the plate is again incubated for 30 minutes and washed. Substrate is then added to the plate, which reacts with the bound insulin conjugated antibody. After a third incubation, the reaction is stopped and the intensity of the generated color is read at 450 nm.