检测方法:吸光度440nm (430-460 nm)
检测范围:0.03 - 200U/L
ABSBio™ Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalysis the interconversion of pyruvate and lactate. LDH is an enzyme that is found in almost all of the body's cells (as well as in bacteria) and is released from cells into the fluid portion of blood (serum or plasma) when cells are damaged or destroyed. Thus, the blood level of LDH is a general indicator of tissue and cellular damage. The level of LDH may also rise in the presence of liver diseases, anemia, leukemia, cancer and severe infections.
The ABS_Bio™ Lactate Dehydrogenase Activity Detection Kit provides a simple, sensitive, one-step high-throughput adaptable colorimetric assay to detect LDH in various samples. In this assay, Lactate dehydrogenase converts lactate and NAD+ into pyruvate and NADH, NADH reduces the tetrazolium salt WST-1 to formazan through the redox activity. The reduced formazan is soluble and its absorption at 440 nm is proportional to the amount of LDH activity in the sample. The kit is supplied with sufficient reagents for 100 tests in 96-well plate assay, linear detection range of 0.03-200 U/L with only 20 µL sample volume.
NADH standard in 96 wells-plate assay (colorimetric)
Series dilute FBS sample in 96 wells-plate assay