Description: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate - H2O2 stimulated
Contents :LF-R0002A: H2O2 stimulated HeLa Cell Lysate 100ug
LF-R0002B: H2O2 unstimulated HeLa Cell Lysate100ug
Concentration: 1 mg/ml
Storage: Stable for 6 months at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing
Product Prep: Cells were cultured for 30 minutes in the presence of 1mM H2O2 (only LF-R0002A - H O stimulated HeLa cell lysate). Then cells were lysed in Lysis buffer (20mM HEPES, pH7.2, 20mM Phosphoglycerate, 150mM NaCl, 10% glycerol, 1% NP-40, 1mM EDTA, 1mM EGTA, 1mM Na3VO4, 5mM NaF, 1mM PMSF, 5ug/ml Pepstatin A, 5ug/ml Aprotinin, 5ug/ml Leupeptin) and diluted with reducing sample buffer (60mM Tris, pH 6.8, 6% glycerol, 2% SDS, 0.2% bromphenolblue, 200mM 2-mercaptoethanol).
Usage: Boil for 5minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Immunoblot analysis.