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Jurkat Cell Lysate (Human T-lymphocyte cell line)
LF-R0007 Abfrontier
2310.00 / 200 ul
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产品说明 / Product Overview:

Description: Jurkat (Human T-lymphocyte cell line) Whole Cell Lysate
Application: WB
Concentration: 1 mg/ml
Storage: Stable for 6 months at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing
Form: Liqid
Product Prep: Cells were lysed in Lysis buffer(20mM HEPES, pH7.2, 20mM Phosphoglycerate, 150mM NaCl, 10% glycerol, 1% NP-40, 1mM EDTA, 1mM EGTA, 1mM Na3VO4, 5mM NaF, 1mM PMSF, 5ug/ml Pepstatin A, 5ug/ml Aprotinin, 5ug/ml Leupeptin) and diluted with reducing sample buffer (60mM Tris, pH 6.8, 6% glycerol, 2% SDS, 0.2% bromphenol blue, 200mM 2- mercaptoethanol).
Usage: Used as a positive Immunoblotting control for many Abfrontier antibodies
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