DetectX® Retinol Binding Protein(RBP) High Sensitivity Enzyme Immunoassay Kit
灵敏度：2.90 ng / mL；
• Use - Kidney Function Measured in 90 MInutes
• Sample - Urine, Dried Blood Spot (DBS)
• Samples/Kit - 41 or 233 in Duplicate
• Stability - Stable 4˚C Liquid Reagents
The DetectX® RBP High Sensitivity ELISA Kit (formerly named Urinary RBP EIA Kit) is designed to measure RBP levels in urine and dried blood spots. This kit uses a native human RBP molecule as a standard. Standards or diluted samples are added to a coated microtiter plate along with a RBP-peroxidase conjugate and the binding reaction initiated by addition of a RBP-specific rabbit antibody. After a 60-minute incubation, the plate is washed and TMB substrate solution added. After 30 minutes the intensity of the generated signal is read at 450nm.
Retinol binding protein (RBP) is from a family of structurally related proteins that bind small hydrophobic molecules such as bile pigments, steroids, odorants, etc. RBP is a 21 kDa highly conserved, single-chain glycoprotein, consisting of 182 amino acids with 3 disulfide bonds, that has a hydrophobic pocket which binds retinol. RBP has been shown to be a useful marker for renal function as it is totally filtered by the glomeruli and reabsorbed by proximal tubules. This has made RBP a tool to study renal function in heart or kidney transplant recipients, type 1 and 2 diabetics, and in people exposed to uranium from mining operations. Measurement of RBP levels has also been useful in detection and characterization of diseases including hypertension and certain cancers, among other conditions.
Sensitivity was calculated by comparing the OD’s for twenty wells run for each of the B0 and standard #5. The detection limit was determined at two (2) standard deviations from the B0 along the standard curve.
Sensitivity was determined as 2.90 ng/mL.
中昊新生（zhscience）是Arbor Assays中国区域总代理，一如既往地为客户提供Arbor Assays高性价比产品，和专业的技术服务。Arbor Assays公司专注于研发高质量的免疫检测试剂盒，产品具有高灵敏度、适应物种广泛等特点，且性价比极高。其中标识“N-Cal™”的试剂盒，产品校准已达至WHO或美国国家标准技术研究院（NIST）参考材料标准。