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Biocytin C2 maleimide生物胞素C2马来酰亚胺
3085 AAT
2145.00 / 5mg
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产品说明 / Product Overview:

生物胞素C2马来酰亚胺(Biocytin C2 maleimide)分子量为494.61。
生物胞素马来酰亚胺容易与生物大分子硫羟基部分反应,形成硫醚偶联物,其十分稳定。这种生物胞素马来酰亚胺需要温和地结合条件,例如,在PH5.5–8.5通常是修饰半胱氨酸残基的最佳PH, 并且无论什么时候,尽量减少暴露在空气中溶液反应,尽可能避免硫羟化底物在空气中氧化。
大多偶联物在室温下形成。然而,温度的升高或降低都可能需要特定的标记反应。这种生物素酰化试剂必须置于无外来硫醇的缓冲液中(比如:6-巯基乙醇、二硫苏糖醇和巯基乙胺)反应。蛋白质或者多肽通过与不含有硫醇基团(SH)的试剂进行硫羟化反应形成生物胞素。
Biocytin maleimide readily reacts with thiol moieties of biopolymers to form thioether conjugate that is quite stable. This biocytin maleimide requires mild conjugation conditions. For example, pH of 5.5–8.5 is usually optimal for modifying cysteine residues, and air exposure of reaction solution should be minimized whenever possible to avoid the air oxidation of thiol substrates. Most conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction. Reactions with this biotinylation reagent should be performed in buffers free of extraneous thiols (such as 6-mercaptoethanol, dithiothreitol and mercaptoethylamine). Proteins or peptides to be biotinylated by thiol-reactive reagents must have a free thiol group (SH) available. 
 


Protocol
               References               MSDS

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