Atrial Natriuretic Peptide(ANP) Enzyme Immunoassay Kit
DetectX® ANP免疫分析试剂盒旨在定量测量血浆，尿液和组织培养基样品中的ANP。90分钟两步分析。该试剂盒提供了ANP标准品以生成标准曲线。 将标准品或稀释的样品吸移到微量滴定板中，将ANP-过氧化物酶结合物添加到孔中的标准品和样品中，通过向每个孔中添加针对ANP的抗体来启动结合反应。 温育一个小时后，洗涤板并加入底物。 底物与结合的ANP-过氧化物酶缀合物反应。 短暂孵育后，停止反应，并在微量滴定板读数器中于450nm下检测所产生颜色的强度。
Use - Quantitate ANP in 90 Minute 2-Step Assay
Sample - Plasma, Urine, or Tissue Culture Media
Samples/Kit - 40 or 232 in Duplicate
Stability - Liquid 4˚C Stable Reagents
The DetectX® ANP Immunoassay kit is designed to quantitatively measure ANP present in plasma, urine and tissue culture media samples. An ANP standard is provided to generate a standard curve. Standards or diluted samples are pipetted into a clear coated microtiter plate. An ANP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to ANP to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound ANP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450nm.