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Cal-520™葡聚糖偶联物,Cal-520™-Dextran Conjugate
20600 AAT
待定 / 1mg
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产品说明 / Product Overview:

Cal-520™葡聚糖偶联物,激发波长:492nm;发射波长:514nm;分子量:3,000。

钙的测定对于许多生物学研究至关重要。荧光探针与钙结合后的光谱响应可以通过荧光显微镜、流式细胞仪、荧光光谱和荧光酶标仪来研究胞内游离钙浓度的变化。

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. Compared to Oregon Green BAPTA-1 dextran, Cal-520 dextran exhibits much lower background and much larger calcium-induced fluorescence enhancement.

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