AAT Bioquest’s one step ReadiUse™ TMB Substrate Solution is optimized for ELISA assays that use horseradish peroxidase (HRP)-labeled conjugates and hydrogen peroxide in microwell plates or test tubes. Our TMB solution allows the following HRP reaction kinetics to be readily followed.
HRP and HRP conjugates facilitate the above reaction in the presence of hydrogen peroxide, turning TMB into its blue oxidized product. The oxidized TMB product has the absorption maximum of 650 nm that can easily be followed with a spectrophotometer. Use of a stop solution enhances sensitivity 2-4 fold and the resulting yellow solution can be read at 450 nm. ReadiUse™ TMB Substrate Solution provides an ultrasensitive quantitative substrate system. The product is extremely stable, it is supplied ready to use with no mixing required.
ReadiUse™ TMB底物溶液 使用说明