Cell Meter™ 荧光法细胞内过氧亚硝酸盐检测试剂盒，适用于流式细胞术。
Peroxynitrite (ONOO¯) is a strong oxidizing species and a highly active nitrating agent. Peroxynitrite is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can damage a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Meanwhile, peroxynitrite can also have protective activities in vivo by contributing to host-defense responses against invading pathogens. Therefore, peroxynitrite is an essential biological oxidant involved in a board ranges of physiological and pathological processes.
Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and understand the role of peroxynitrite in biological systems. In order to address this unmet need, AAT Bioquest’s Cell Meter™ Fluorimetric Intracellular Peroxynitrite (ONOO¯) Assay Kit provides a sensitive tool to monitor ONOO¯ level in living cells.
AAT Bioquest’s DAX-J2™ PON Green is developed as an excellent fluorescent probe, which can specifically react with intercellular ONOO¯ to generate a bright green fluorescent product. This kit is optimized for flow cytometry.
Cell Meter™ 荧光法细胞内过氧亚硝酸盐检测试剂盒（适用于流式细胞术）使用说明书