Protein Concentration: 0.20 mg/ml (vortex before apply for gel electrophoresis)
Size/Physical State: 100 μl (20 applications of 5 μl each). ApoCs Standard is a readyto-use format - no mixing, heating or reducing required. It is pre-reduced and contains reducing reagents in the loading buffer. The protein standards resolve into bands with 6.6 kD for apoCI, 8.5 kD for apoCII, and 8.7 kD for ApoCIII; ApoAII appears as weak visible band between ApoCI and ApoCII.
Expiration: One year from the date of purchasing
Source: From purified ApoCs and ApoAII; fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens.
Use: The Un-Stained ApoCs Standard allows you to check the result of your gel run and to judge western transfer efficiency. The attached gel was running with 4 µl of each for lane 1 (ApoCs) and lane 2 (Std Mix2) on an Invitrogen 18% SDS-Gel.
Buffer: In loading buffer consists of Tris-HCl, MeSH, SDS, glycerol, and bromophenol.
Storage: -20ºC for short and long-term storage. Aliquot to avoid repeated freezing and thawing.