细胞划痕愈合检测试剂盒
The ABSBio™ Scratch Wound Healing Assay Kit provides a simple, sensitive, one-step colorimetric and fluorimetric assay for cell migration and proliferation. In this assay, a “wound gap” in a cell monolayer is created by scratching, and the “healing” of this gap by cell migration and growth towards the center of the gap is monitored and quantitated. Factors that alter the motility and/or growth of the cells can lead to increased or decreased rate of “healing” of the gap. The Cell proliferation and migration rates can be determined using manual fixing and microscopic imaging.
The kit is supplied with sufficient reagents for 100 tests in 24-well or 48-well plate assay. It could easily be modified for a high-throughput assay.
间隙距离可以使用Photoshop或ImageJ等软件进行定量评估 (http://rsb.info.nih.gov/ij/download.html)
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