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Collagenase Type V 胶原酶Ⅴ
货号:H0083 | 规格:100mg | 价格:¥0.00 | 品牌:BMASSAY
BMASSAY® 提供Collagenase Type V 胶原酶Ⅴ。BMASSAY®提供众多高纯度的生化试剂,包括抗生素、氨基酸、维生素、酶和辅酶、蛋白质、缓冲液、染色剂、层析介质、动植物激素、糖 碳水化合物、酸和盐、碱基 核酸及其衍生物等。
胶原酶,即:胶原蛋白酶,又称:梭菌肽酶A,英文名称:Collagenase,来源于溶组织梭菌,Collagenase from Clostridium histolyticum,CAS:9001-12-1, 性状:棕色或棕褐色结晶性粉末。

EINECS编号232-582-9;Enzyme Commission(EC)号:3.4.24.3;MDL编号:MFCD00130830。

胶原酶V型,Collagenase Type V, 活性:≥1 FALGPA units/mg solid, >125 CDU/mg solid.
可溶性:TESCA缓冲液(50mM TES,0.36mM氯化钙,pH7.4):可溶性1-2mg / mL

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

应用
Collagenase from Clostridium histolyticum has been used in a study to investigate the degradation of collagen by the cariogenic bacteria, Streptococcus mutans. Collagenase from Clostridium histolyticum has also been used in a study to investigate the survivability of collagen micronetworks in the presence of collagenase.

Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, and lung epithelial tissues among several others. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes. The product has been used to digest forearm skin biopsies from Parkinson disease patients to grow human skin fibroblast primary cultures. The enzyme from Sigma has been used for rodent islet isolation in a study that assayed glucokinase functions.

Storage:−20°C.
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