链霉蛋白酶E，英文名称：Pronase E

又称：链丝菌蛋白酶；链霉菌蛋白酶；Actinase E

CAS：9036-06-0，EINECS编号 232-909-5，MDL number MFCD00132092。

Grade……………… for molecular biology；
from……………… Streptomyces griseus；
Type ………………type XIV;
Unit…………………≥3.5 units/mg solid。

蛋白酶 来源于灰色链霉菌, Protease from Streptomyces griseus。

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease from Streptomyces griseus is a mixture of at least three proteolytic activities including an extracellular serine protease. Serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

This product is a mixture of at least three caseinolytic activities and one aminopeptidase activity.The caseinolytic enzymes were named as Streptomyces griseus Protease A, Streptomyces griseus Protease B and Streptomyces griseus Trypsin. This product may be used when extensive or complete degradation of protein is required. This protease mixture is highly nonspecific and can digest casein to the extent of >70% as mono-amino acids.

链霉蛋白酶E(Pronase E)为浅黄褐色粉末,为从灰色链霉菌(Streptomyces griseus)中分离出的一种非特异蛋白水解酶(Protease)的专有名称。

用途：生化研究，裂开蛋白质的肽键。

储存: −20°C。