蛋白酶K(Proteinase K)，来源于白色念珠菌(Tritirachium album)。

CAS号 39450-01-6, EINECS编号 254-457-8, EC Number 3.4.21.64, MDL number MFCD00132129.

Synonym: Endopeptidase K.

Purity……………..≥90%;
Activity………..≥40 units/mg protein;
DNase,RNase & Nickase…………None detected.

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

One unit is defined as the amount of enzyme that will liberate 1.0 μmol tyrosine from casein per min at 37°C, pH 7.5. pH optimum 7.5 - 12.0.

蛋白酶K是一种切割活性较广的丝氨酸蛋白酶，它切割脂族氨基酸和芳香族氨基酸的羧基端肽键。用于制备脉冲电泳的染色体DNA，蛋白质印迹以及去除DNA和RNA制备中的核酸酶。溶于水(10 mg/ml)

孵育温度为55至65℃，理想孵育温度为58℃，孵育时间为15分钟至48小时；理想孵育时间为2小时。

冷冻（-20℃）保存,避免反复冻融，有效期12月。