The Ludger EB10 cartridges contain a 10 mg bed of Ludger Electron Interaction Resin (EIR). This unique material contains very flat regions of pi-orbital electrons that interact with hydrophilic organic compounds including glycans.

The resin can capture a wide range of glycans from complex mixtures while salts and certain detergents pass through. The glycans are then eluted from the cartridge using a simple solvent system.

The cartridge housing is constructed of polypropylene and is compatible with many automated sample preparation systems.

Binding Capacity: Each LudgerCleanTM EB10 cartridge can typically bind up to 50 μg of O- or N-linked glycans.

Number of Samples: Ludger EB10 cartridges are designed for single use only.

Suitable Samples: A wide range of glycans can be purified. These include N-linked and O-linked type oligosaccharides, tri-saccharides and larger structures.

The cartridges are not suitable either for monosaccharides or disaccharides which are generally bound too weakly for efficient purification or for large linear poly-sialylated glycans which can be bound very tightly to the resin.

Glycan samples must be applied to the cartridges in solutions that are substantially aqueous.

Structural Integrity: No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.

Binding efficiency: >95% for most glycans

Binding Selectivity: Essentially stoichiometric binding and elution for most complex glycan mixtures.

Storage: Store at room temperature in the dark. Protect from sources of heat, light, and moisture. The cartridges are stable for at least two years as supplied.

Shipping: The product can be shipped at ambient temperature.

Handling: Ensure that any glass, plastic ware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Safety: Please read the Safety Data Sheet (SDS) for all chemicals used.

All processes involving hazardous reagents should be performed using appropriate personal safety protection – eyeglasses, chemically resistant gloves (e.g. nitrile), etc. – and where appropriate in a laboratory fume hood.