统一服务热线:400-888-0207 在线咨询 | 微信联系
首页 > 产品 > 产品类型 > 试剂盒 > 免疫检测试剂盒
Creatinine Urinary Detection Kit (2 Plate)肌酐尿液检测试剂盒
货号:K002-H1 | 规格:2x96 well plate | 价格:¥2913.75 | 品牌:Arbor Assays
肌酐尿液检测试剂盒是由北京中昊新生科技有限公司提供Arbor Assays的产品具有高灵敏、适应物种广泛等特点,且性价比极高。 北京中昊新生科技有限公司是美国Arbor Assays中国代理商,专注于提供高质量的免疫检测试剂盒。

DetectX® Urinary Creatinine Detection kit
DetectX®肌酐尿液检测试剂盒用于定量测量尿液样本中的肌酐。NIST校准的肌酐标准品用于标准化分析。
已验证的样本类型:人,猴,狗和大鼠尿液;
灵敏度为0.019 mg / dL。

如要测量血清或血浆样品中的肌酐,请参考DetectX®血清肌酐检测试剂盒,货号KB02-H1/KB02-H2KB02-H1D



• Use - Urine Volume
• Sample - Urine
• Samples/Kit - 88 or 472 in Duplicate
• Calibrated - N-Cal Kit, NIST-Calibrated
• Stability - Liquid 4°C Stable Reagents
       
The DetectX® Urinary Creatinine Detection kits are designed to quantitatively measure creatinine in urine samples. A NIST calibrated creatinine standard is used to standardize the assay. Samples are pipetted into a clear microtiter plate and the color generating reaction is initiated with the supplied no-mix DetectX Creatinine Reagent, which is pipetted into each well. After a 30 minute incubation at room temperature the color is read at 490nm.

Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine.  Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine.

典型标准曲线
        
灵敏度
Sensitivity was calculated by comparing the OD’s for twenty wells run for each of the zero and standard #7. The detection limit was determined at two (2) standard deviations from the zero along the standard curve.
Sensitivity was determined as 0.019 mg/dL.

检测下限
The Limit of Detection for the assay was determined in a similar manner by comparing the OD’s for twenty wells run for each of the zero standard and a low concentration human urine sample. 
Limit of Detection was determined as 0.037 mg/dL.

说明书

相关产品