SOD活性检测试剂盒

DetectX®Superoxide Dismutase(SOD)活性检测试剂盒用于定量检测各种样品中的SOD活性。该试剂盒可以检测所有类型的SOD活性，包括Cu/Zn,Mn和FeSOD类型。

检测类型:Activity Assay

样本类型:Serum, Plasma, Cells, Tissue Culture Media, Erythrocytes

灵敏度:0.044 U/mL

适应物种:人

检测时间:20分钟

样品数/板:40 in Duplicate

读数:Colorimetric, 450 nm

DetectX^®超氧化物歧化酶(SOD)活性试剂盒提供牛红细胞SOD标准品以生成测定的标准曲线，并且应从标准曲线中读取所有样品。 样品用特殊颜色的样品稀释液稀释后加入孔中。 加入底物，然后加入黄嘌呤氧化酶试剂，并在室温下孵育20分钟。 黄嘌呤氧化酶在氧气存在下产生超氧化物，它将检测试剂中的无色底物转化为黄色产物。 在450nm处读取有色产物。 样品中SOD含量的增加会导致超氧化物浓度的降低和黄色产物的减少。 结果以每毫升的SOD活性单位表示。

Short-lived and highly reactive oxygen species (ROS) such as O2-· (superoxide), ·OH (hydroxyl radical), and H2O2 (hydrogen peroxide) are continuously generated in vivo. The cellular levels of ROS are controlled by antioxidant enzymes and small molecule antioxidants. The major antioxidant enzymes, superoxide dismutases (SODs), including copper-zinc superoxide dismutase (Cu/ZnSOD), manganese superoxide dismutase (MnSOD), and extracellular superoxide dismutase (EC-SOD). All play a critical roles in scavenging O2-·. Decreased SOD activity results in elevated level of superoxide which in turn leads to decreased NO and increased peroxynitrite concentrations.

The major intracellular SOD is a 32-kDa copper and zinc containing homodimer (Cu/Zn SOD). The mitochondrial SOD (MnSOD) is a manganese-containing 93-kDa homotetramer that is synthesized in the cytoplasm and translocated to the inner matrix of mitochondria. EC-SOD is the primary extracellular SOD enzyme and is highly expressed in many organs. Increased SOD activity levels are seen in Downs Syndrome, while decreased activity is seen in diabetes, Alzheimer’s disease, rheumatoid arthritis, Parkinson’s disease, uremic anemia, atherosclerosis, some cancers, and thyroid dysfunction.

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Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。

北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供SOD活性检测试剂盒,超氧化物歧化酶活性检测试剂盒最新现货优惠信息。