Filgrastim (GCSF) Pharmacokinetic ELISA Kit<br>适用物种:human<br><br>样本类型:Serum,Plasma<br><br>检测范围:9.375ng/mL - 500 ng/mL<br><br>最低检出限:9.375 ng/mL<br><br>测定时间:2.5 hours<br><br>检测格式:Direct sandwich ELISA<br><br>检测原理:<br>This assay employs the indirect sandwich enzyme immunoassay technique. Anti-GCSF is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. GCSF present in biological matrices is bound by the immobilized anti- GCSF antibody. After washing away any unbound substances, tagged anti-GCSF secondary antibody is added to the wells. After washing, diluted detection reagent is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of GCSF present in test samples. The color development is stopped and the intensity of the color is measured.<br><br>检测方法:Peroxidase / OD450 <br><br> 靶抗原:granulocyte colony stimulating factor (G-CSF)<br><br> 应用:granulocyte colony stimulating factor in biological matrices<br><br> 包被板类型:Strip<br><br> 样品体积:15ul<br><br> 特异性:GCSF<br><br> 检测精度(批内差异,批间差异):< 25% for ULOQ and LLOQ and <20% for the remaining concentrations<br><br> 防腐剂:None<br><br> Gene ID:1440<br><br> 试剂盒组分:<br>Coated microtiter plate, 96 wells (1x8 strips) 1 Calibrator diluent 1.8ml Calibrator (1mg/ml) 12μl 20X wash buffer 25ml Assay buffer 50ml 1000X secondary antibody 1000X detection reagent 17μl TMB 12ml TMB stop solution 12ml<br><br> 储存温度和稳定性:Stable at -20°C for 1 year<br><br> 背景描述:<br>Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream<br><br> 检测程序:<br>This assay employs the indirect sandwich enzyme immunoassay technique. Anti-GCSF is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. GCSF present in biological matrices is bound by the immobilized anti- GCSF antibody. After washing away any unbound substances, tagged anti-GCSF secondary antibody is added to the wells. After washing, diluted detection reagent is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of GCSF present in test samples. The color development is stopped and the intensity of the color is measured.<br><br> 样本收集:<br>This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.<br><br> 样品制备:<br>Dilute calibrators and test samples 1/50 with assay buffer (for example add 5µL of prepared calibrator or sample to 245µL of assay buffer). Mix well. Do not store diluted samples.<br><br> 试剂制备:<br>Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. <br>1. Wash Buffer (1X) Preparation: Dilute wash buffer

concentrate with ultra-pure water 1/20 before use

(for example add 25mL concentrate to 475mL ultrapure water). Mix well.

<br>2. Secondary Antibody (1X) Preparation: Dilute secondary

antibody with assay buffer 1/1000 before use (for

example add 12μl to 12mL of assay buffer). Mix well.

<br>3. Detection Reagent (1X) Preparation: Dilute detection

reagent with assay buffer 1/1000 before use (for

example add 12μl concentrate to 12ml of assay buffer).

Mix well.

<br>4. Calibrator Preparation: Dilute the calibrator from 1mg/ml

down to 5µg/ml by pipetting 5µL of calibrator stock into

995µL assay buffer. Label "Cal. Int." Mix well. Prepare

calibrators with concentrations ranging from 500 ng/ml

to 9.375 ng/ml. The following is an example calibrator

curve.<br><br> 结果计算:<br>1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. <br>2. The concentration of the unknowns can be back calculated directly from this standard curve using the absorbance value for each sample. <br>3. Any sample diluted more or less than the standard series will need additional data correction. For example, if the sample is diluted 1/50, then the concentration will be calculated by dividing by 2 due to the calibrators being 2 times more diluted. Similarly, if the sample is diluted 1/500, then the concentration will be calculated by multiplying by a correction factor of 5 due to the calibrators being 5 times more concentrated.<br><br> <br><br>AffinityImmuno致力于为生物药物研究提供高品质ELISA试剂盒和单克隆抗体，同时提供定制鸡多克隆抗体的服务。<br>

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