人IL-10 ELISA试剂盒/人白细胞介素10 ELISA试剂盒用于定量检测天然或重组人IL-10。

适用样品：血清，血浆及细胞培养基

检测范围：32–5000 pg/ml

IL-10 (Interleukin-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine.

ABSbio™ Human IL-10 ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Human IL-10 in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for high-throughput users. Human IL-10 containing samples or standards and a biotinylated detection antibody directed against Human IL-10 are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to

produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-10 in samples.

The kit is designed to quantitatively measure natural and/or recombinant Human IL-10 within the range of 32–5000 pg/ml. The kit is sufficient to assay human IL-10 in approximately 96 ELISA plate wells.