ABSbio™大鼠IL-1β ELISA试剂盒/大鼠白介素1 Beta ELISA试剂盒,用于以夹心ELISA形式定量检测天然或重组大鼠IL-1β,检测范围为25–4000 pg/ml,适用样品:血清,血浆及细胞培养基。

Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis.

ABSbio™ Rat IL-1β ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Rat IL-1β in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for high-throughput users. Rat IL-1β containing samples or standards and a biotinylated detection antibody directed against Rat IL-1β are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IL-1β in samples. The kit is designed to quantitatively measure natural and/or recombinant Rat IL-1β within the range of 25–4000 pg/ml. The kit is sufficient to assay Rat IL-1β in approximately 96 ELISA plate wells.