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Human Lactoferrin ELISA Kit
货号:HLTFKT | 规格:5 kits | 价格:¥0.00 | 品牌:Molecular Innovations
Human Lactoferrin ELISA Kit是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-870、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Human lactoferrin (aka lactotransferrin or growth-inhibiting protein 12) is an 82.4kDa 691 amino acid glycoprotein which carries 2 ferric ions per molecule and is the main iron binding protein in milk. Lactoferrin has multiple physiological functions including iron absorption regulation and antibacterial, antiviral, and antiparasitic activity. Degranulated neutrophils are the primary source of plasma lactoferrin which is increased during inflammation and bacterial infection. The sensitive quantitative measurement of human lactoferrin in human plasma, serum, milk, and cell culture samples is easily performed with this 96 well strip format ELISA kit. The normal human concentration of lactoferrin is 100 ng/ml in plasma and 5.3 mg/ml in milk. The assay measures human lactoferrin in the 0.1-100 ng/ml range. Samples giving human lactoferrin levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:100-1:500 dilution for plasma or serum and and 1:200,000 dilution for breast milk is suggested for best results. Human lactoferrin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated anti-human lactoferrin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of human lactoferrin and is measured along with the test samples. Color development is proportional to the concentration of total lactoferrin in the samples. All reagents and standards are provided in these ELISA kits.
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