MagAttract® PowerSoil® DNA KF Kit用于使用自动处理和液体处理系统从土壤中免提分离DNA。

For hands-free isolation of DNA from soil using automated processing and liquid-handling systems

The MagAttract PowerSoil DNA KF Kit is optimized for use with the Thermo Scientific™ KingFisher® Flex and KingFisher Duo platforms.

The MagAttract PowerSoil DNA KF Kit can be used for automated isolation of microbial DNA from all types of soil as well as other environmental samples high in humic acids, like compost, sediment and manure. The kit can be used to process as many as 384 samples of up to 0.25 grams each. The method employs Inhibitor Removal Technology® (IRT) to remove PCR inhibitors released during the extraction process. Additionally, a novel, proprietary magnetic bead system is used to isolate nucleic acids from the IRT-treated lysate without binding residual contaminants. The result is inhibitor-free DNA that is ready to use in demanding downstream applications, including PCR, qPCR and next-generation sequencing (NGS).

Principle and procedure
Environmental samples are added to a 96 well bead beating plate for rapid and thorough homogenization. Cell lysis occurs by a combination of mechanical and chemical methods.Humic acids are removed using IRT. Prepared lysates are transferred to the KingFisher Flex or KingFisher Duo platforms where total genomic DNA is captured on specialized magnetic beads in the presence of buffers that avoid the use of chaotropic salts and ethanol. DNA is washed on the beads and then eluted using a 10 mM Tris (pH 8) buffer.

Quantification of DNA using PicoGreen® will yield values approximately 15% lower than the actual yield due to the presence of residual wash solution in the DNA. The wash solution does not inhibit PCR or interfere with NGS.