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Human PAI-1 (N-terminal cysteine LRP non-binding stable mutant)
货号:NTCYSCPAI-R76E | 规格:1.0 mg | 价格:¥0.00 | 品牌:Molecular Innovations
Human PAI-1 (N-terminal cysteine LRP non-binding stable mutant)是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-1480、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
This stable form of PAI-1 contains an N-terminal cysteine mutation in addition to the four stabilization point mutations. A substitution of Glutamic Acid for Arginine at position 76 greatly decreases the binding of this Human PAI-1 mutant to the low density lipoprotein receptor-related protein (LRP). The protein is supplied in buffer with 1mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
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