DBH (Dopamine-beta-Hydroxylase) Antibody  多巴胺b羟化酶抗体

The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 – 1/4000 dilution in rat brainstem, cerebellum and adrenal medulla.

The above image is of DBH positive neurons in rat brainstem,  vibratome sectioned, ABC / DAB detection.

Using Western blot of purified DBH the antiserum detects a triplet at approximately 72-74 kD. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

照片描述: IHC image of neurons staining for DBH in the rat brainstem. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

宿主: Rabbit

规格/体积:  100 µL

性状: Lyophilized Whole Serum

Species Reactivity: Bird, Cat, Ferret, Finch, Guinea Pig, Hamster, Hatchetfish, Human, Ilyanassa Obsoleta (Sea Snail), Monkey, Mouse, Pig, Quail, Rat, Sparrow, Starling, Steer (Cattle), Turkey, Turtle, Zebra Finch

别名:  DBM; DOPO; Dopamine beta-monooxygenase, anti-DBH

RRID:  AB_572229

免疫原: Bovine DBH

Gene Symbol:  DBH