Sulfo-NHS-Biotin (Sulfosuccinimidyl biotin) is a water soluble biotinylation reagent used in Biotin-Streptavidin binding reactions. Sulfo-NHS-Biotin is soluble in 4.4mg/mL water (may be slightly hazy). Sulfo-NHS-Biotin is amine reactive and has a spacer arm length of 13.5 Å. The Sulfo-NHS-Biotin biotinylation reagent is ideal for biotinylating antibody or protein that can then be used for protein detection or immobilization when used in conjunction with streptavidin and Immobilized Streptavidin. Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. The water insoluble version of Sulfo-NHS-Biotin is NHS-Biotin.

Sulfo-NHS-Biotin and the "long chain" Sulfo-NHS-LC-Biotin both have the same chemical functionality, but the latter will provide a longer spacer arm more capable of overcoming steric hindrances that may be present between the labeled protein(s) and streptavidin (ProteoChem product # e7105-5mg). Often, the best results are determined by empirically testing each reagent for your specific application.

Molecular Weight: 443.43

Spacer Length: 13.5 Å

CAS Number: 119616-38-5

Alternative Names: Biotin-NHS, Water-Soluble; Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt; Sulfosuccinimidobiotin; Sulfo NHS Biotin; Sulfosuccinimidyl biotin; N-hydroxysulfosuccinimidyl biotin; N-hydroxysulfosuccinimido biotin