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TC Protector
货号:TCP-001 | 规格:100 ml | 价格:¥0.00 | 品牌:KAC
TC Protector/细胞冻存保护剂是一种细胞冻存液,不含动物源性成分或蛋白质,不粘稠,不形成气泡,用于需要添加血清的细胞,无血清细胞和无蛋白质细胞。
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TC Protector/TC保护剂是一种细胞冷冻保护剂(Cell Cryoprotective agent),不含任何动物源性成分或蛋白质,是化学定义的。可用于需要添加血清的细胞、无血清的细胞和无蛋白质的细胞。

TC Protector适用于细胞系细胞和正常细胞,以及ES细胞,成体干细胞和人iPS细胞。不粘稠,也不会形成气泡。因此,具有极好的可用性。


特征

  • 根本不含动物源性成分,是化学定义的。

  • 不含任何血清或蛋白质。

  • 在冰箱中储存,取出后可立即使用。

  • 粘性很小,可用性极好。

  • 不需要特殊设备,例如程序冷冻机。可以直接存储于−80ºC。


冷冻方法

1. In case of adhesive cells, media should be changed at 80% confluence and be frozen on the next day.

In case of floating cells, media change (or media supplementation) is conducted and should be frozen on the next day.

*In order to freeze cells in a good condition, it is important to use cells from the logarithmic growth phase.

*Cells that have reached confluence or overgrown result in a decreased survival rate after freezing.

2. Cells are harvested according to the standard procedure and are centrifuged at 1,500 rpm for 1 minute.

3. It is diluted with TC Protector to achieve 5×105~1×107 cell/mL.

*Cell suspension is maintained in ice water when cell counts are measured.

*Since the appropriate cell number varies depending on cells, a pilot study must be conducted in advance. In general, 1×106 cells/mL is the target cell count.

4. Cells are stored at −80ºC in a Deep Freezer and are transferred into liquid nitrogen on the next day.


解冻方法

Ampules are placed in a warm water bath at 37ºC, and it is quickly thawed until it forms small ice mass.

Cell suspension is collected into a spitz tube and about 10× media is added. Then, it is centrifuged at 1,000–1,500 rpm for 1–2 minutes.

Cell pellet is checked and supernatant is discarded. Cells are seeded in accordance with the standard method.

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