Sections of mouse liver, stained with monoclonal antibody F4/80 Normal liver with positive Kupffer cells
Liver of a mouse, 2 days after injection with clodronate-liposomes. Kupffer cells have been depleted
Sections of mouse spleen stained for acid phosphatase Normal spleen with positive splenic macrophages
Spleen of a mouse 2 days after injection with clodronate liposomes. Only few macrophages are left
所有的参考 ALL REFERENCES：使用氯膦酸脂质体的已发表研究的完整列表
Here are a few useful references of the first publications on the depletion of phagocytic cells in various organs and tissues by use of clodronate liposomes. Further, the list includes reviews about the mechanism of clodronate liposome mediated apoptosis of macrophages, its applications in macrophage research, possible therapeutic applications and preparation of clodronate liposomes.
First publication of the approach
Van Rooijen, N., & Van Nieuwmegen, R. (1984). Elimination of phagocytic cells in the spleen after intravenous injection of liposome-encapsulated dichloromethylene diphosphonate. Cell and tissue research, 238(2), 355-358.
Application in research and therapeutics
van Rooijen, N., & van Kesteren-Hendrikx, E. (2002). Clodronate liposomes: perspectives in research and therapeutics. Journal of liposome research, 12(1-2), 81-94.
Elimination and blocking of macrophages in general
Rooijen and Annemarie Sanders, N. (1997). Elimination, blocking, and activation of macrophages: three of a kind?. Journal of leukocyte biology, 62(6), 702-709.
Mechanism macrophage apoptosis mediated by clodronate liposome
Van Rooijen, N., Sanders, A., & van den Berg, T. K. (1996). Apoptosis of macrophages induced by liposome-mediated intracellular delivery of clodronate and propamidine. Journal of immunological methods, 193(1), 93-99.
Depletion of Kupffer cells in liver
Van Rooijen, N. I. C. O., & Sanders, A. (1996). Kupffer cell depletion by liposome‐delivered drugs: comparative activity of intracellular clodronate, propamidine, and ethylenediaminetetraacetic acid. Hepatology, 23(5), 1239-1243.
Depletion of macrophages in spleen
Van Rooijen, N., Van Nieuwmegen, R., & Kamperdijk, E. W. A. (1985). Elimination of phagocytic cells in the spleen after intravenous injection of liposomeencapsulated dichloromethylene diphosphonate. Virchows Archiv B, 49(1), 375.
Van Rooijen, N., Kors, N., & Kraal, G. (1989). Macrophage subset repopulation in the spleen: differential kinetics after liposome‐mediated elimination. Journal of leukocyte biology, 45(2), 97-104.
Van Rooijen, N., Kors, N., Vd Ende, M., & Dijkstra, C. D. (1990). Depletion and repopulation of macrophages in spleen and liver of rat after intravenous treatment with liposome-encapsulated dichloromethylene diphosphonate. Cell and tissue research, 260(2), 215-222.
Depletion of macrophages in lymph nodes
Delemarre, F. G. A., Kors, N., Kraal, G., & Van Rooijen, N. (1990). Repopulation of macrophages in popliteal lymph nodes of mice after liposome‐mediated depletion. Journal of leukocyte biology, 47(3), 251-257.
Depletion of alveolar macrophages in lung
Thepen, T., Van Rooijen, N., & Kraal, G. (1989). Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice. Journal of Experimental Medicine, 170(2), 499-509.
Depletion of peritoneal macrophages
Biewenga, J., van der Ende, M. B., Krist, L. F., Borst, A., Ghufron, M., & van Rooijen, N. (1995). Macrophage depletion in the rat after intraperitoneal administration of liposome-encapsulated clodronate: depletion kinetics and accelerated repopulation of peritoneal and omental macrophages by administration of Freund’s adjuvant. Cell and tissue research, 280(1), 189-196.
Depletion of testis macrophages
Bergh, A., Damber, J. E., & Van Rooijen, N. (1993). Liposome-mediated macrophage depletion: an experimental approach to study the role of testicular macrophages in the rat. Journal of endocrinology, 136(3), 407-NP.
Depletion of phagocytic synovial lining cells in joints
Blom, A. B., van Lent, P. L., Holthuysen, A. E., van der Kraan, P. M., Roth, J., van Rooijen, N., & van den Berg, W. B. (2004). Synovial lining macrophages mediate osteophyte formation during experimental osteoarthritis. Osteoarthritis and cartilage, 12(8), 627-635.
Depletion of perivascular and meningeal macrophages in the central nervous system
Polfliet, M. M., Goede, P. H., van Kesteren-Hendrikx, E. M., van Rooijen, N., Dijkstra, C. D., & van den Berg, T. K. (2001). A method for the selective depletion of perivascular and meningeal macrophages in the central nervous system. Journal of neuroimmunology, 116(2), 188-195.