抗人CD3(UCHT-1)抗体
货号：ICH1002-100mg
品牌：ichorbio
产品特性
Bulk Anti-Human CD3 (UCHT-1) Antibody is manufactured in an cGMP compliant facility.
内毒素: <1.0 EU/mg as determined by the LAL method
纯度: >95% by SDS-PAGE and HPLC
应用: Flow Cytometry, Immunoprecipitation, Western Blot, IHC (Frozen), CyTOF, Functional Assays.
| 规格 | 价格 |
|---|---|
| 100mg | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：抗人CD3(UCHT-1)抗体
别名：抗人CD3抗体, Bulk Anti-Human CD3 (UCHT-1) Antibody, Anti-Human CD3 Antibody, CD3 epsilon chain, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain
宿主：Mouse
应用：Flow Cytometry, Immunoprecipitation, Western Blot, IHC (Frozen), CyTOF, Functional Assays
亚型：Mouse IgG1
克隆：UCHT-1
浓度：≥ 2.0 mg/ml
免疫原：Unknown
反应性：Human
特异性：Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) recognizes Human CD3. anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein. The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR)
存储溶液：0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
储存条件：anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
推荐同型对照（Isotype Control）：Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0； 体内抗体缓冲液pH7.4； 体内抗体缓冲液pH8.0
靶点（Target）: CD3
UniProt: ：P07766
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.
背景资料（Background）: ：Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias. The UCHT-1 clone is recognized in the human leukocyte differentiation antigen workshop III 471.
纯度（Purity）: ：>95% by SDS-PAGE and HPLC
内毒素（Endotoxin）: ：<1.0 EU/mg as determined by the LAL method
聚集（Aggregation）: ：Aggregation level ≤ 5%
应用说明（Application Notes）: ：Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin. Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）: ：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件: ：Blue ice
返回列表
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0； 体内抗体缓冲液pH7.4； 体内抗体缓冲液pH8.0
靶点（Target）: CD3
UniProt: ：P07766
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.
背景资料（Background）: ：Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias. The UCHT-1 clone is recognized in the human leukocyte differentiation antigen workshop III 471.
纯度（Purity）: ：>95% by SDS-PAGE and HPLC
内毒素（Endotoxin）: ：<1.0 EU/mg as determined by the LAL method
聚集（Aggregation）: ：Aggregation level ≤ 5%
应用说明（Application Notes）: ：Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin. Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）: ：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件: ：Blue ice
