抗小鼠CD28(37.51)体内抗体-低内毒素
货号：ICH1055-5mg
品牌：ichorbio
产品特性
Anti-Mouse CD28 (37.51) In Vivo Antibody - Low Endotoxin is manufactured in an cGMP compliant facility.
内毒素: ≤ 1.0 EU/mg as determined by the LAL method
纯度: >95% by SDS-PAGE and HPLC
应用: Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, IHC (Frozen).
| 规格 | 价格 |
|---|---|
| 5mg | ￥咨询 |
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服务热线：400-860-6200
详细介绍
产品名称：抗小鼠CD28(37.51)体内抗体-低内毒素
别名：抗小鼠CD28体内抗体, 抗小鼠CD28抗体-低内毒素, Anti-Mouse CD28 (37.51) In Vivo Antibody - Low Endotoxin, Anti-Mouse CD28 In Vivo Antibody ,Anti-Mouse CD28 Antibody - Low Endotoxin
宿主：Syrian Hamster
应用：Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, IHC (Frozen)
亚型：Syrian Hamster IgG
克隆：37.51
浓度：1.0 - 5.0 mg/ml
免疫原：C57BL/6 mouse T-cell lymphoma EL-4
反应性：Mouse
特异性：Anti-CD28 In Vivo Antibody - Low Endotoxin (37.51) recognizes the CD28 co-stimulatory receptor, a ligand of CD80 (B7-1) and CD86 (B7-2). CD28 is important for transmitting co-stimulatory signals for optimal T-cell activation.
存储溶液：20 mM Histidine pH 6.0 containing 150 mM NaCl. NOTE: A buffer exchange into PBS for conjugation and MES for functional assays may be required depending on your application.
储存条件：anti-CD28 In Vivo Antibody - Low Endotoxin (37.51) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0；
体内抗体缓冲液pH7.4；
体内抗体缓冲液pH8.0
靶点（Target）: CD28
UniProt: ：P31041
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：Anti-CD28 is present on most thymocytes, CD4+, CD8+ T-cells. The 37.51 antibody augments proliferation and IL-2 production by activated T-cells. Clone 37.51 antibody has been shown to stimulate the proliferation and cytokine production by activated T and NK cells and provide a costimulatory signal for CTL induction.
背景资料（Background）: ：CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. It is expressed on the cell surface as disulfide-linked homodimer or as monomer. CD28 is the receptor for B7.1 (CD80) and B7.2 (CD86). When activated by Toll-like receptor ligands, the B7.1 expression is upregulated in antigen presenting cells (APCs). The B7.2 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. Stimulation through CD28 in addition to the TCR can provide a potent co-stimulatory signal to T cells for the production of various interleukins (IL-2 and IL-6 in particular). CD28 possesses an intracellular domain with several residues that are critical for its effective signalling. CD28 also contains two proline-rich motifs that are able to bind SH3-containing proteins. CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-gamma.
纯度（Purity）: ：>95% by SDS-PAGE and HPLC
内毒素（Endotoxin）: ：≤ 1.0 EU/mg as determined by the LAL method
聚集（Aggregation）: ：Aggregation level ≤ 5%
应用说明（Application Notes）: ：Functional Assay: Anti-Mouse CD28 (clone 37.51) has been reported in costimulation of T cells In vitro and In vivo. Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）: ：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件: ：Blue ice
返回列表
靶点（Target）: CD28
UniProt: ：P31041
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：Anti-CD28 is present on most thymocytes, CD4+, CD8+ T-cells. The 37.51 antibody augments proliferation and IL-2 production by activated T-cells. Clone 37.51 antibody has been shown to stimulate the proliferation and cytokine production by activated T and NK cells and provide a costimulatory signal for CTL induction.
背景资料（Background）: ：CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. It is expressed on the cell surface as disulfide-linked homodimer or as monomer. CD28 is the receptor for B7.1 (CD80) and B7.2 (CD86). When activated by Toll-like receptor ligands, the B7.1 expression is upregulated in antigen presenting cells (APCs). The B7.2 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. Stimulation through CD28 in addition to the TCR can provide a potent co-stimulatory signal to T cells for the production of various interleukins (IL-2 and IL-6 in particular). CD28 possesses an intracellular domain with several residues that are critical for its effective signalling. CD28 also contains two proline-rich motifs that are able to bind SH3-containing proteins. CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-gamma.
纯度（Purity）: ：>95% by SDS-PAGE and HPLC
内毒素（Endotoxin）: ：≤ 1.0 EU/mg as determined by the LAL method
聚集（Aggregation）: ：Aggregation level ≤ 5%
应用说明（Application Notes）: ：Functional Assay: Anti-Mouse CD28 (clone 37.51) has been reported in costimulation of T cells In vitro and In vivo. Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）: ：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件: ：Blue ice
