抗小鼠PD-1(29F.1A12)体内抗体-超低内毒素
货号：ICH1091UL-100mg
品牌：ichorbio
产品特性
Anti-Mouse PD-1 (29F.1A12) In Vivo Antibody - Ultra-Low Endotoxin is manufactured in an cGMP compliant facility.
内毒素: ≤ 0.5 EU/mg as determined by the LAL method
纯度: >98% by SDS-PAGE and HPLC
应用: Flow Cytometry, Western Blot, Blocking, IHC (Frozen).
| 规格 | 价格 |
|---|---|
| 100mg | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：抗小鼠PD-1(29F.1A12)体内抗体-超低内毒素
别名：抗小鼠PD-1体内抗体, 抗小鼠PD-1抗体-超低内毒素, Anti-Mouse PD-1 (29F.1A12) In Vivo Antibody - Ultra-Low Endotoxin, Anti-Mouse PD-1 In Vivo Antibody ,Anti-Mouse PD-1 Antibody - Ultra-Low Endotoxin, Programmed cell death protein 1, Pdcd1, CD279
宿主：Rat
应用：Flow Cytometry, Western Blot, Blocking, IHC (Frozen)
亚型：Rat IgG2a Kappa
克隆：29F.1A12
浓度：≥ 2.0 mg/ml
免疫原：PD-1 cDNA followed by PD-1-Ig fusion protein
反应性：Mouse
特异性：Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
存储溶液：0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
储存条件：anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
推荐同型对照（Isotype Control）：Rat IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [1-1] (ICH2244UL)
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0； 体内抗体缓冲液pH7.4； 体内抗体缓冲液pH8.0
靶点（Target）: PD-1
UniProt：Q02242
纯化方法（Purification method）: This monoclonal antibody was purified using Protein G
抗原分布（Antigen Distribution）：Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells
背景资料（Background）：Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.
纯度（Purity）：>98% by SDS-PAGE and HPLC
内毒素（Endotoxin）：≤ 0.5 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 1%
IMPACT病原体检测（IMPACT Pathogen Test）：We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
应用说明（Application Notes）：Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
返回列表
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0； 体内抗体缓冲液pH7.4； 体内抗体缓冲液pH8.0
靶点（Target）: PD-1
UniProt：Q02242
纯化方法（Purification method）: This monoclonal antibody was purified using Protein G
抗原分布（Antigen Distribution）：Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells
背景资料（Background）：Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.
纯度（Purity）：>98% by SDS-PAGE and HPLC
内毒素（Endotoxin）：≤ 0.5 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 1%
IMPACT病原体检测（IMPACT Pathogen Test）：We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
应用说明（Application Notes）：Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
