抗小鼠IFNAR1(MAR1-5A3)体内抗体-低内毒素
货号：ICH1122-100mg
品牌：ichorbio
产品特性
Anti-Mouse IFNAR1 (MAR1-5A3) In Vivo Antibody - Low Endotoxin is manufactured in an cGMP compliant facility.
内毒素: ≤ 1.0 EU/mg as determined by the LAL method
纯度: >95% by SDS-PAGE and HPLC
应用: Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, ELISA.
| 规格 | 价格 |
|---|---|
| 100mg | ￥咨询 |
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详细介绍
产品名称：抗小鼠IFNAR1(MAR1-5A3)体内抗体-低内毒素
别名：抗小鼠IFNAR1体内抗体, 抗小鼠IFNAR1抗体-低内毒素, Anti-Mouse IFNAR1 (MAR1-5A3) In Vivo Antibody - Low Endotoxin, Anti-Mouse IFNAR1 In Vivo Antibody, Anti-Mouse IFNAR1 Antibody - Low Endotoxin, Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Type I interferon receptor 1, Ifar, Ifnar
宿主：Mouse
应用：Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, ELISA
亚型：Mouse IgG1
克隆：MAR1-5A3
浓度：≥ 2.0 mg/ml
免疫原：This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1.
反应性：Mouse
特异性：Anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) recognizes the extracellular domain of the IFNAR1 subunit of the mouse IFN-alpha / beta receptor.
存储溶液：0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
储存条件：anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. NOTE: Do not freeze as this antibody at -20°C as this clone will sometimes precipitate over long period of time.
推荐同型对照（Isotype Control）：Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0； 体内抗体缓冲液pH7.4； 体内抗体缓冲液pH8.0
靶点（Target）: IFNAR1
UniProt：P33896
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：IFNAR1 and IFNAR2 are coexpressed on nearly all cells.
背景资料（Background）：The antibody when prepared specifically for In vivo functional assays blocks type I IFN receptor signaling both In vitro and In vivo without depleting IFNAR1 bearing cells. This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1. IFNAR1 and IFNAR2 are coexpressed on nearly all cells and make up the heterodimeric receptor that binds all type I IFNs (IFN alpha and beta). Type I IFNs are a family of cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor and autoimmune responses In vivo.
纯度（Purity）：>95% by SDS-PAGE and HPLC
内毒素（Endotoxin）：≤ 1.0 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 5%
应用说明（Application Notes）：Blocking: Clone MAR1-5A3 has a short half-life, basically because every cell expresses the IFNAR1 receptor and the receptor recycles very rapidly. And, if you want to block function in vivo, you need to be sure that all of the receptors are blocked continually in all compartments. Therefore, you need a large in vivo loading dose (2.5 mg/mouse) to saturate all the binding sites in vivo and then maintain a high enough level to keep them saturated. For in vivo blocking studies we recommend give a loading dose of 2.5 mg/mouse and follow with a weekly dose of 0.5 mg/mouse. The half-live following a 2.5 mg loading dose is about 5 days. [However, if you only inject a low dose of 250 micrograms, then the half life is 1.5 days – because you haven’t saturated the mouse]. Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
返回列表
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0； 体内抗体缓冲液pH7.4； 体内抗体缓冲液pH8.0
靶点（Target）: IFNAR1
UniProt：P33896
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：IFNAR1 and IFNAR2 are coexpressed on nearly all cells.
背景资料（Background）：The antibody when prepared specifically for In vivo functional assays blocks type I IFN receptor signaling both In vitro and In vivo without depleting IFNAR1 bearing cells. This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1. IFNAR1 and IFNAR2 are coexpressed on nearly all cells and make up the heterodimeric receptor that binds all type I IFNs (IFN alpha and beta). Type I IFNs are a family of cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor and autoimmune responses In vivo.
纯度（Purity）：>95% by SDS-PAGE and HPLC
内毒素（Endotoxin）：≤ 1.0 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 5%
应用说明（Application Notes）：Blocking: Clone MAR1-5A3 has a short half-life, basically because every cell expresses the IFNAR1 receptor and the receptor recycles very rapidly. And, if you want to block function in vivo, you need to be sure that all of the receptors are blocked continually in all compartments. Therefore, you need a large in vivo loading dose (2.5 mg/mouse) to saturate all the binding sites in vivo and then maintain a high enough level to keep them saturated. For in vivo blocking studies we recommend give a loading dose of 2.5 mg/mouse and follow with a weekly dose of 0.5 mg/mouse. The half-live following a 2.5 mg loading dose is about 5 days. [However, if you only inject a low dose of 250 micrograms, then the half life is 1.5 days – because you haven’t saturated the mouse]. Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
