抗小鼠CD27(RM27-3E5)体内抗体-超低内毒素
货号：ICH1185UL-50mg
品牌：ichorbio
产品特性
Anti-Mouse CD27 (RM27-3E5) In Vivo Antibody - Ultra-Low Endotoxin is manufactured in an cGMP compliant facility.
内毒素: ≤ 0.5 EU/mg as determined by the LAL method
纯度: >98% by SDS-PAGE and HPLC
应用: Functional Assays, Flow Cytometry, ImmunoPrecipitation.
| 规格 | 价格 |
|---|---|
| 50mg | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：抗小鼠CD27(RM27-3E5)体内抗体-超低内毒素
别名：抗小鼠CD27体内抗体, 抗小鼠CD27抗体-超低内毒素, Anti-Mouse CD27 (RM27-3E5) In Vivo Antibody - Ultra-Low Endotoxin, Anti-Mouse CD27 In Vivo Antibody, Anti-Mouse CD27 Antibody - Ultra-Low Endotoxin, CD27L receptor, Tnfrsf7
宿主：Rat
应用：Functional Assays, Flow Cytometry, ImmunoPrecipitation
亚型：Rat IgG1 Kappa
克隆：RM27-3E5
浓度：≥ 5.0 mg/ml
免疫原：Mouse CD27-human IgG1 Fc fusion protein
反应性：Mouse
特异性：RM27-3E5 activity is directed against mouse CD27
存储溶液：This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
储存条件：This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
推荐同型对照（Isotype Control）：Rat IgG1 Isotype Control for In Vivo – Low Endotoxin (GL113) [ICH2246]
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0； 体内抗体缓冲液pH7.4； 体内抗体缓冲液pH8.0
靶点（Target）: CD27
UniProt：P41272
纯化方法（Purification method）: This monoclonal antibody was purified using Protein G
抗原分布（Antigen Distribution）：CD27 is expressed on T cells, B cells, and NK cells
背景资料（Background）：CD27 is a glycosylated, type I transmembrane protein of the TNFR superfamily that is constitutively expressed on the surface of progenitor and naïve T cells as well as subsets of natural killer (NK) and B cells. CD27 is an immune-checkpoint receptor and ligation with CD70 results in co-stimulatory effects that enhance primary, memory, and recall T cell responses. CD27 signaling has been targeted for the development of viral, autoimmune, and anti-tumor immunotherapies. RM27-3E5 (rat IgG2a) was raised against recombinant mouse CD27-human IgG1 Fc fusion protein and purified from ascites using protein G columns. RM27-3E5 enhances anti-CD3-stimulated T cell proliferation when immobilized in vitro and augments ovalbumin-specific cytotoxic T lymphocyte (CTL) induction when administered in vivo. RM27-3E5 is an agonistic antibody and has been found to support the control of both established lung metastases and subcutaneous tumors. An anti-tumor effect was also observed with RM27-3E5 against EG7/CD27(+) and EG7/CD27(−) tumors, with the effect mediated mainly by T cells with a minor contribution by NK cells. RM27-3E5 exerts its potent anti-tumor effect by inducing a tumor-specific CTL response. RM27-3E5 has potential utility as a non-depleting agonistic anti-CD27 mAb to enhance anti-tumor immunity
纯度（Purity）：>98% by SDS-PAGE and HPLC
内毒素（Endotoxin）：≤ 0.5 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 1%
IMPACT病原体检测（IMPACT Pathogen Test）：We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp.
Mycoplasma pulmonis
Sendai virus
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV1-5)
Theiler's murine encephalomyelitis virus
Murine norovirus
Reovirus 3
Mouse rotavirus
Ectromelia virus
Lymphocytic choriomeningitis virus
Polyoma virus
Lactate dehydrogenase-elevating virus
Mouse adenovirus (MAD1, MAD2)
Mouse cytomegalovirus
K virus
Mouse thymic virus
Hantaan virus
Corynebacterium bovis
Corynebacterium spp. (HAC2)
应用说明（Application Notes）：Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
返回列表
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0； 体内抗体缓冲液pH7.4； 体内抗体缓冲液pH8.0
靶点（Target）: CD27
UniProt：P41272
纯化方法（Purification method）: This monoclonal antibody was purified using Protein G
抗原分布（Antigen Distribution）：CD27 is expressed on T cells, B cells, and NK cells
背景资料（Background）：CD27 is a glycosylated, type I transmembrane protein of the TNFR superfamily that is constitutively expressed on the surface of progenitor and naïve T cells as well as subsets of natural killer (NK) and B cells. CD27 is an immune-checkpoint receptor and ligation with CD70 results in co-stimulatory effects that enhance primary, memory, and recall T cell responses. CD27 signaling has been targeted for the development of viral, autoimmune, and anti-tumor immunotherapies. RM27-3E5 (rat IgG2a) was raised against recombinant mouse CD27-human IgG1 Fc fusion protein and purified from ascites using protein G columns. RM27-3E5 enhances anti-CD3-stimulated T cell proliferation when immobilized in vitro and augments ovalbumin-specific cytotoxic T lymphocyte (CTL) induction when administered in vivo. RM27-3E5 is an agonistic antibody and has been found to support the control of both established lung metastases and subcutaneous tumors. An anti-tumor effect was also observed with RM27-3E5 against EG7/CD27(+) and EG7/CD27(−) tumors, with the effect mediated mainly by T cells with a minor contribution by NK cells. RM27-3E5 exerts its potent anti-tumor effect by inducing a tumor-specific CTL response. RM27-3E5 has potential utility as a non-depleting agonistic anti-CD27 mAb to enhance anti-tumor immunity
纯度（Purity）：>98% by SDS-PAGE and HPLC
内毒素（Endotoxin）：≤ 0.5 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 1%
IMPACT病原体检测（IMPACT Pathogen Test）：We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp.
Mycoplasma pulmonis
Sendai virus
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV1-5)
Theiler's murine encephalomyelitis virus
Murine norovirus
Reovirus 3
Mouse rotavirus
Ectromelia virus
Lymphocytic choriomeningitis virus
Polyoma virus
Lactate dehydrogenase-elevating virus
Mouse adenovirus (MAD1, MAD2)
Mouse cytomegalovirus
K virus
Mouse thymic virus
Hantaan virus
Corynebacterium bovis
Corynebacterium spp. (HAC2)
应用说明（Application Notes）：Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
