抗人CD18(IB4)抗体
货号：ICH1189-100mg
品牌：ichorbio
产品特性
Bulk Anti-Human CD18 (IB4) Antibody is manufactured in an cGMP compliant facility.
内毒素: <1.0 EU/mg as determined by the LAL method
纯度: >95% by SDS-PAGE
应用: Functional Assays, Flow Cytometry, IF Microscopy.
| 规格 | 价格 |
|---|---|
| 100mg | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：抗人CD18(IB4)抗体
别名：抗人CD18抗体, Bulk, Anti-Human CD18 (IB4) Antibody, Anti-Human CD18 Antibody, Integrin beta-2, integrin beta chain, beta 2, ITGB2
宿主：Mouse
应用：Functional Assays, Flow Cytometry, IF Microscopy
亚型：Mouse IgG2a Kappa
克隆：1B4
浓度：≥ 2.0 mg/ml
免疫原：Human peripheral blood lymphocytes
反应性：Human
特异性：Anti-CD18 antibody (clone 1B4) activity is directed against human CD18 and can also stain rabbit bone marrow leukocytes.
存储溶液：Sterile, preservative-free, solution in PBS. BSA and Azide free.
储存条件：This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
推荐同型对照（Isotype Control）：Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0；体内抗体缓冲液pH7.4；体内抗体缓冲液pH8.0
靶点（Target）: CD18
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：Depending on its heterodimeric configuration, CD18 is expressed in leukocytes, monocytes, macrophages, some lymphocytes, polymorphonuclear lymphocytes (PMNs), natural killer cells, neutrophils, granulocytes, certain lymphocytic tumor cell lines, macrophage foam cells, activated platelets, and splenic red pulp macrophages.
背景资料（Background）：Murine (m) 1B4 was humanized (h) by the CDR-grafting method to create an anti-CD18 antibody with similar avidity, specificity, and efficacy as the murine antibody. In rhesus monkeys, h1B4 is less immunogenic than m1B4 and elicits only anti-idiotypic antibodies. Additionally, h1B4 and m1B4 exhibit equivalent staining specificity and intensity in rabbit bone marrow leukocytes.For humanization, human VH and VL FR were chosen based on high identity with their corresponding m1B4 FR3. Once grafted with the m1B4 CDR, the human VH and VL FR were expressed as fusion proteins with the IgG4 and Cκ human constant regions, respectively.The h1B4 monoclonal antibody has been used in multiple studies of adhesion in different model systems and to measure the expression of CD18 in B-ALL cell lines.
纯度（Purity）：>95% by SDS-PAGE
内毒素（Endotoxin）：<1.0 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 5%
应用说明（Application Notes）：Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
返回列表
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0；体内抗体缓冲液pH7.4；体内抗体缓冲液pH8.0
靶点（Target）: CD18
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：Depending on its heterodimeric configuration, CD18 is expressed in leukocytes, monocytes, macrophages, some lymphocytes, polymorphonuclear lymphocytes (PMNs), natural killer cells, neutrophils, granulocytes, certain lymphocytic tumor cell lines, macrophage foam cells, activated platelets, and splenic red pulp macrophages.
背景资料（Background）：Murine (m) 1B4 was humanized (h) by the CDR-grafting method to create an anti-CD18 antibody with similar avidity, specificity, and efficacy as the murine antibody. In rhesus monkeys, h1B4 is less immunogenic than m1B4 and elicits only anti-idiotypic antibodies. Additionally, h1B4 and m1B4 exhibit equivalent staining specificity and intensity in rabbit bone marrow leukocytes.For humanization, human VH and VL FR were chosen based on high identity with their corresponding m1B4 FR3. Once grafted with the m1B4 CDR, the human VH and VL FR were expressed as fusion proteins with the IgG4 and Cκ human constant regions, respectively.The h1B4 monoclonal antibody has been used in multiple studies of adhesion in different model systems and to measure the expression of CD18 in B-ALL cell lines.
纯度（Purity）：>95% by SDS-PAGE
内毒素（Endotoxin）：<1.0 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 5%
应用说明（Application Notes）：Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
