抗小鼠CD16.2(9E9)体内抗体-超低内毒素
货号：ICH1194UL-25mg
品牌：ichorbio
产品特性
Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Ultra-Low Endotoxin is manufactured in an cGMP compliant facility.
内毒素: ≤ 0.5 EU/mg as determined by the LAL method
纯度: >98% by SDS-PAGE and HPLC
应用: Blocking, Flow Cytometry.
| 规格 | 价格 |
|---|---|
| 25mg | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：抗小鼠CD16.2(9E9)体内抗体-超低内毒素
别名：抗小鼠CD16.2体内抗体, 抗小鼠CD16.2抗体-超低内毒素, Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Ultra-Low Endotoxin, Anti-Mouse CD16.2 In Vivo Antibody- Ultra-Low Endotoxin, Anti-Mouse CD16.2 Antibody- Ultra-Low Endotoxin, FcγRIV
宿主：Armenian Hamster
应用：Blocking, Flow Cytometry
亚型：Armenian Hamster IgG
克隆：9E9
浓度：≥ 2.0 mg/ml
免疫原：9E9 was produced by immunizing Armenian hamsters with an FcγRIV-IgG1 fusion protein consisting of the extracellular domain of FcγRIV fused to a mouse IgG1 Fc portion (D265A-variant deficient in Fc-receptor binding). Splenic B cells were then fused to a mouse fusion partner, and hybridoma clones were screened for binding to CHO-K1-FcγRIV cells expressing FcγRIV.
反应性：Mouse
特异性：9E9 activity is primarily directed against mouse CD16.2 / FcγRIV but can also bind and block FcγRIII in vivo.
存储溶液：Sterile, preservative-free, solution in PBS. BSA and Azide free.
储存条件：This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
推荐同型对照（Isotype Control）：Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251)
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0；体内抗体缓冲液pH7.4；体内抗体缓冲液pH8.0
靶点（Target）: CD16.2
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：FcγRIV is expressed on the cell membrane of splenic and bone marrow dendritic cells, monocytes, and macrophages as well as peripheral blood monocytes, neutrophils, thioglycollate-elicited macrophages, and myeloid cells. FcγRIV is absent from lymphoid populations, T cells, B cells, NK cells, and other granulocytes.
背景资料（Background）：According to surface plasmon resonance, 9E9 has strong reactivity to FcγRIV as well as low level binding to FcγRII and FcγRIII. In vivo, 9E9 binds and blocks FcγRIII only when 9E9 first binds FcγRIV on the same effector cell, resulting in concurrent inhibition of FcγRIII and FcγRIV. Native 9E9 binds to FcγRII and FcγRIII via the Fc.Blocking studies with 9E9 show that FcγRIV is necessary for IgG2a and IgG2b mediated platelet clearance in vivo. Additionally, blocking FcγRIV with 9E9 reduces B-cell depletion. 9E9 also interferes with immune complex binding to FcγRIV and can block FcγRIII on macrophages and neutrophils.
纯度（Purity）：>98% by SDS-PAGE and HPLC
内毒素（Endotoxin）：≤ 0.5 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 1%
IMPACT病原体检测（IMPACT Pathogen Test）：We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Hamster parvovirus
Lymphocytic choriomeningitis
Minute virus of mice
Theiler's murine encephalomyelitis virus
Sendai virus
Reovirus 3
Hantaan virus
应用说明（Application Notes）：Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
返回列表
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0；体内抗体缓冲液pH7.4；体内抗体缓冲液pH8.0
靶点（Target）: CD16.2
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：FcγRIV is expressed on the cell membrane of splenic and bone marrow dendritic cells, monocytes, and macrophages as well as peripheral blood monocytes, neutrophils, thioglycollate-elicited macrophages, and myeloid cells. FcγRIV is absent from lymphoid populations, T cells, B cells, NK cells, and other granulocytes.
背景资料（Background）：According to surface plasmon resonance, 9E9 has strong reactivity to FcγRIV as well as low level binding to FcγRII and FcγRIII. In vivo, 9E9 binds and blocks FcγRIII only when 9E9 first binds FcγRIV on the same effector cell, resulting in concurrent inhibition of FcγRIII and FcγRIV. Native 9E9 binds to FcγRII and FcγRIII via the Fc.Blocking studies with 9E9 show that FcγRIV is necessary for IgG2a and IgG2b mediated platelet clearance in vivo. Additionally, blocking FcγRIV with 9E9 reduces B-cell depletion. 9E9 also interferes with immune complex binding to FcγRIV and can block FcγRIII on macrophages and neutrophils.
纯度（Purity）：>98% by SDS-PAGE and HPLC
内毒素（Endotoxin）：≤ 0.5 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 1%
IMPACT病原体检测（IMPACT Pathogen Test）：We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Hamster parvovirus
Lymphocytic choriomeningitis
Minute virus of mice
Theiler's murine encephalomyelitis virus
Sendai virus
Reovirus 3
Hantaan virus
应用说明（Application Notes）：Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
