Exosome分离及Exosomal RNA的纯化
2026-04-28
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Exosome分离及Exosomal RNA( 含miRNA) 的纯化 |
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exoEasy Maxi Kit 从血浆、血清、细胞培养上清及其他生物体液中分离完整的 exosome 及其他细胞外囊泡 |
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exoRNeasy Maxi/Midi Kit 采用亲和性膜离心柱，可从 exosomes 和来自血清、血浆、细胞培养上清液或尿液的其他细胞外微泡中高效分离 RNA。 Maxi 规格的分离柱可处理多达 4 ml 血清 / 血浆、16 ml 尿液或 32 ml 细胞培养上清液，因此能够可靠检测低丰度 RNA。 该试剂盒能够快速、便利的获得结果，十分适用于敏感的下游应用。 |
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| Intact vesicles are eluted from the exoEasy membrane with higher purity compared with ultracentrifugation. Scanning electron microscopy (SEM; 20,000x magnification) was performed on a solubilized pellet from ultracentrifugation of pre-filtered (0.8 µm) plasma compared to an exoEasy eluate. Both preparations contain vesicle-shaped structures with an expected size range from 50–200 nm (white arrows; scale bar 200 nm). The preparation from ultracentrifugation also includes many smaller, unidentified structures that do not match the expected shape and size of extracellular vesicles. |
Characterization of extracellular vesicles isolated using the exoEasy Maxi Kit by Nanoparticle Tracking Analysis (NTA). (A) HeLa cells were maintained in serum-free medium for 48 h before harvesting culture supernatants. The exoEasy Maxi Kit was used to isolate EVs from 2 ml of supernatant that had been pre-filtered using a 0.8 µm filter. EVs were diluted 1:100 and characterized using the Nanosight NS300 instrument (Malvern, UK). (B) EVs were also isolated from 1 ml of EDTA plasma (measured at 1:200). Mean particle diameters were reported as 212 nm and 214 nm, respectively, which is within the expected range for a mixed population of exosomes and other extracellular vesicles. Red outline indicates variation between 5 repeat measurements. |
| 产品 |
描述 |
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| exoEasy Maxi Kit |
用于纯化来自血浆、血清和细胞培养上清液中的外泌体和 其他细胞外囊泡 (EVs) | 76064 |
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| exoRNeasy Maxi Kit (50) exoRNeasy Midi Kit (50) exoRNeasy Serum/Plasma Starter Kit (20) |
从尿液、血清、血浆、脑脊液、细胞培养上清及其他体液 中提取外泌体 RNA |
77164 77144 77023 |
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| Reagent UI (1ml) | 搭配 exoRNeasy Maxi/Midi Kit 使用，需单独购买 | 77900 |
