产品特性
比色法检测（检测波长：480 - 492 nm）
检测范围：21.9～4378 µmol/L（基于铜离子还原能力）
可在5分钟内检测样品的抗氧化能力
用于检测血清、食品和饮料样品中的总抗氧化能力
| 规格 | 价格 |
|---|---|
| 96 wells | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：总抗氧化能力(PAO)检测试剂盒
别名：Test kit for Potential Anti Oxidant；Potential Anti Oxidant Assay Kit；PAO Assay Kit；Total Antioxidant Capacity Assay Kit；Test kit for Total Antioxidant Capacity；总抗氧化能力检测试剂盒；潜在抗氧化剂检测试剂盒；PAO检测试剂盒；T-AOC检测试剂盒
应用：Human and animal serum samples, foods and beverage samples.
检测方法：Colormetric assay(detection: 480 - 492 nm)
检测范围：21.9 - 4378 µmol/L (cupric ion reducing power)
储存条件：室温（10 - 25°C）
抗氧化检测：
氧化应激在多种疾病及衰老过程中起着重要作用。控制氧化应激有望有助于预防疾病和延缓衰老。氧化应激是由活性氧（ROS）与抗氧化防御系统之间的失衡引起的。为准确评估氧化应激状态，测定ROS水平、氧化损伤程度及抗氧化活性至关重要。近年来，作为功能性食品的抗氧化剂因能清除ROS而备受关注。
检测原理：
本PAO检测试剂盒是一种简便快速的抗氧化能力检测方法。该方法基于铜离子的还原反应（Cu++还原为Cu+），可在5分钟内检测样品的抗氧化能力。PAO不仅可以检测亲水性抗氧化剂（如维生素C、谷胱甘肽），还可以检测疏水性抗氧化剂（如维生素E）。适用于血清、食品及饮料样本的总抗氧化能力评估。
检测流程：将样品与Cu++溶液混合后，抗氧化剂将Cu++还原形为Cu+。还原生成的Cu+与显色溶液（Bathocuproine）结合反应，在480 - 490 nm波长处检测吸光度，根据生成的Cu+计算总抗氧化能力。
试剂盒组分：
Standard (Uric acid powder): 1 vial
Sample diluent: 1 bottle
Cu++ solution: 1 bottle
Stop solution: 1 bottle
Micro titer plate: 1 plate (96 wells)
需用户自备
A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are prepared with distilled water only).
检测步骤：
1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 µL of Sample Diluent, and add 10 µL of standards or diluted samples.
3) Pour 200 µL of mixture to Micro titer plate. Use 200 µL of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 µL of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 µL of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 - READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (µmol/L).
1mM of uric acid = 2189 µmol/L (copper reducing power)
[注意事项]：该总抗氧化能力(PAO)检测试剂盒仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
氧化应激在多种疾病及衰老过程中起着重要作用。控制氧化应激有望有助于预防疾病和延缓衰老。氧化应激是由活性氧（ROS）与抗氧化防御系统之间的失衡引起的。为准确评估氧化应激状态，测定ROS水平、氧化损伤程度及抗氧化活性至关重要。近年来，作为功能性食品的抗氧化剂因能清除ROS而备受关注。
检测原理：
本PAO检测试剂盒是一种简便快速的抗氧化能力检测方法。该方法基于铜离子的还原反应（Cu++还原为Cu+），可在5分钟内检测样品的抗氧化能力。PAO不仅可以检测亲水性抗氧化剂（如维生素C、谷胱甘肽），还可以检测疏水性抗氧化剂（如维生素E）。适用于血清、食品及饮料样本的总抗氧化能力评估。
检测流程：将样品与Cu++溶液混合后，抗氧化剂将Cu++还原形为Cu+。还原生成的Cu+与显色溶液（Bathocuproine）结合反应，在480 - 490 nm波长处检测吸光度，根据生成的Cu+计算总抗氧化能力。
试剂盒组分：
Standard (Uric acid powder): 1 vial
Sample diluent: 1 bottle
Cu++ solution: 1 bottle
Stop solution: 1 bottle
Micro titer plate: 1 plate (96 wells)
需用户自备
A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are prepared with distilled water only).
检测步骤：
1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 µL of Sample Diluent, and add 10 µL of standards or diluted samples.
3) Pour 200 µL of mixture to Micro titer plate. Use 200 µL of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 µL of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 µL of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 - READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (µmol/L).
1mM of uric acid = 2189 µmol/L (copper reducing power)
[注意事项]：该总抗氧化能力(PAO)检测试剂盒仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
