铁(Fe)检测试剂盒(亚硝基-PSAP法)
货号：CFE-010
品牌：JaICA
产品特性
Quick & Easy to use.
Species independent!
No toxic substance!
Highly sensitive, stable and suitable for high-throughput testing!
For cell lysates, serum, plasma and wide variety of biological samples.
| 规格 | 价格 |
|---|---|
| 100 tests | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：铁(Fe)检测试剂盒(亚硝基-PSAP法)
别名：Iron Colorimetric Assay Kit (Nitroso-PSAP Method)；Iron Assay Kit；Fe Colorimetric Assay Kit；Fe Assay Kit；铁（Fe）检测试剂盒；铁含量检测试剂盒；铁比色法检测试剂盒；铁检测试剂盒
应用：Colorimetric: 740-760 nm
特异性：Specific to Fe2+ and Fe3+.
检测方法：Nitroso-PSAP Method
样本类型：Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. Metal chelatos, for example EDTA may interfere with this assay.
检测范围：10-1,000 µg/dL
储存条件：2 - 8°C (避免冷冻).
产品优势：
◆ 适用各种物种样本!
◆ 不含任何有毒物质!
◆ 高灵敏度、稳定性强，适用于高通量测试!
◆ 使用快速便捷。适用于细胞裂解液、血清、血浆以及多种生物样本。
产品描述：
Iron is one of the most important elements, which function as enzyme cofactor. Iron in the blood is bound with transferrin and transported throughout the body to synthesize globin proteins such as myoglobin and hemoglobin. Iron is crucial for synthesis of oxygen-transport protein. Its deficiency causes iron deficiency anemia, chronic hemorrhagic anemia and infectious anemia.
检测原理：
This product is a direct colorimetric assay kit without deproteinization of the sample. Dissociated iron from the transferrin-iron complex by weakly acid buffer and reduced by means of reductant (:Ferric→Ferrous). Ferrous ions give a blue colored complex with Nitroso-PSAP (as chromogen). The intensity of the colored complex is proportional to the iron concentration in the sample. The colour intensity is proportional to the amount of iron present in the sample. Absorbance of the Fe2+-complex is measured at 750nm. Wavelength range of sensitivity: 740 ~ 760nm.
样本处理：
Serum/ Plasma: Insoluble substances in serum and plasma samples should be removed by filtration or centrifugation. EDTA-plasma cannot be used.
Urine (24 hour pooled urine)/ Biological fluid: Add 6M HCl to the sample and adjust pH 1.5-3.0 (e.g. 5-10μL 6M HCl/1mL of lysate). Centrifuge at 6,000 rpm for 15 minutes. Collect the supernatant and use it for assay.
Tissue: Add 5% TCA solution, voltex for 1 minute and incubate for 30 minutes at 4-8°C. Centrifuge at 6,000 rpm for 15 minutes. Collect the supernatant and use it for assay.
Note: Sample pH should be between pH 2 and pH 8.
试剂盒组分：
• R-1缓冲液（即用型）：16 mL ×1瓶
• R-2缓冲液（即用型）：7 mL ×1瓶
• R-3螯合显色剂（即用型）：0.7 mL ×1瓶
• 铁标准品（200 µg/dL，即用型）：1.5 mL ×1瓶
[注意事项]：该铁（Fe）比色法检测试剂盒（亚硝基-PSAP法）仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
◆ 适用各种物种样本!
◆ 不含任何有毒物质!
◆ 高灵敏度、稳定性强，适用于高通量测试!
◆ 使用快速便捷。适用于细胞裂解液、血清、血浆以及多种生物样本。
产品描述：
Iron is one of the most important elements, which function as enzyme cofactor. Iron in the blood is bound with transferrin and transported throughout the body to synthesize globin proteins such as myoglobin and hemoglobin. Iron is crucial for synthesis of oxygen-transport protein. Its deficiency causes iron deficiency anemia, chronic hemorrhagic anemia and infectious anemia.
检测原理：
This product is a direct colorimetric assay kit without deproteinization of the sample. Dissociated iron from the transferrin-iron complex by weakly acid buffer and reduced by means of reductant (:Ferric→Ferrous). Ferrous ions give a blue colored complex with Nitroso-PSAP (as chromogen). The intensity of the colored complex is proportional to the iron concentration in the sample. The colour intensity is proportional to the amount of iron present in the sample. Absorbance of the Fe2+-complex is measured at 750nm. Wavelength range of sensitivity: 740 ~ 760nm.
样本处理：
Serum/ Plasma: Insoluble substances in serum and plasma samples should be removed by filtration or centrifugation. EDTA-plasma cannot be used.
Urine (24 hour pooled urine)/ Biological fluid: Add 6M HCl to the sample and adjust pH 1.5-3.0 (e.g. 5-10μL 6M HCl/1mL of lysate). Centrifuge at 6,000 rpm for 15 minutes. Collect the supernatant and use it for assay.
Tissue: Add 5% TCA solution, voltex for 1 minute and incubate for 30 minutes at 4-8°C. Centrifuge at 6,000 rpm for 15 minutes. Collect the supernatant and use it for assay.
Note: Sample pH should be between pH 2 and pH 8.
试剂盒组分：
• R-1缓冲液（即用型）：16 mL ×1瓶
• R-2缓冲液（即用型）：7 mL ×1瓶
• R-3螯合显色剂（即用型）：0.7 mL ×1瓶
• 铁标准品（200 µg/dL，即用型）：1.5 mL ×1瓶
[注意事项]：该铁（Fe）比色法检测试剂盒（亚硝基-PSAP法）仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
