不饱和铁结合力(UIBC)检测试剂盒(Bathophenanthroline)
货号：CBC-800
品牌：JaICA
产品特性
Bathophenanthroline Chromogenic method
适用各种物种
不含任何有毒物质
适配微孔板检测系统
操作步骤简便. 适用于检测血清、血浆样本。
| 规格 | 价格 |
|---|---|
| 100 tests | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：不饱和铁结合力(UIBC)检测试剂盒(Bathophenanthroline)
别名：MC Reagent UIBC Assay Kit (Bathophenanthroline)；Unsaturated Iron Binding Capacity Assay Kit；UIBC检测试剂盒；不饱和铁结合力检测试剂盒；不饱和铁结合力（UIBC）检测试剂盒；UIBC试剂盒；不饱合铁结合力测定试剂盒
应用：Colorimetric: 540-550 nm
特异性：Detection of unsaturated iron binding capacity (UIBC) by measuring a proportional difference between Fe2+ and Fe3+.
检测方法：Bathophenanthroline Chromogenic method
检测范围：10-800 µg/dL
储存条件：2 - 8°C (避免冷冻).
产品描述：
Iron is an important element in organism. Many important enzymes use iron as a cofactor. And iron is an indispensable component to generate protein which transports oxygen. Iron is transported in blood by transferrin. 30% of the transferrin is combined with Fe3+ in blood, and the remainder exists as the free transferrin.
TIBC(Total iron binding capacity) = UIBC(Unsaturated iron binding capacity) + Serum iron. TIBC level changes in blood disorder, hepatic disease, tumor, and inflammation. UIBC level is increased in patients with iron deficiency. Decreased levels are seen in patients with infection disease, malignant, nephrosis syndrome, and low proteinosis.
检测原理：
MC Reagent Biochemical Assay Series UIBC assay kit utilizes the chromogen bathophenanthroline to bind Fe2+. Transferrin in serum is saturated by the addition of buffer which known concentration of Fe3+.And unbound Fe3+ in the buffer is reduced by a reducing agent. Generated Fe2+ react with athophenanthroline and form a pink colored complex. The difference between the resulting change in the measured absorbance and the absorbance from the total amount added to serum is proportional to the quantity bound to transferrin. So UIBC can be calculated by subtract unbound ferric iron from the concentration of the reagent.
血清/血浆样本处理：
需通过过滤或离心去除血清和血浆样本中的不溶性物质。
EDTA抗凝血浆不可使用。
检测前所有试剂需平衡至室温
试剂盒组分：
• R-1缓冲液（铁浓度80μg/dL）：20mL ×1瓶（即用型）
• R-2螯合显色剂：3mL ×1瓶（即用型）
需用户自备：
• 蒸馏水
• 微量移液器及吸头
• 透明平底96孔板
• 酶标仪（主波长546nm）
[注意事项]：该不饱和铁结合力（UIBC）检测试剂盒(Bathophenanthroline)仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
Iron is an important element in organism. Many important enzymes use iron as a cofactor. And iron is an indispensable component to generate protein which transports oxygen. Iron is transported in blood by transferrin. 30% of the transferrin is combined with Fe3+ in blood, and the remainder exists as the free transferrin.
TIBC(Total iron binding capacity) = UIBC(Unsaturated iron binding capacity) + Serum iron. TIBC level changes in blood disorder, hepatic disease, tumor, and inflammation. UIBC level is increased in patients with iron deficiency. Decreased levels are seen in patients with infection disease, malignant, nephrosis syndrome, and low proteinosis.
检测原理：
MC Reagent Biochemical Assay Series UIBC assay kit utilizes the chromogen bathophenanthroline to bind Fe2+. Transferrin in serum is saturated by the addition of buffer which known concentration of Fe3+.And unbound Fe3+ in the buffer is reduced by a reducing agent. Generated Fe2+ react with athophenanthroline and form a pink colored complex. The difference between the resulting change in the measured absorbance and the absorbance from the total amount added to serum is proportional to the quantity bound to transferrin. So UIBC can be calculated by subtract unbound ferric iron from the concentration of the reagent.
血清/血浆样本处理：
需通过过滤或离心去除血清和血浆样本中的不溶性物质。
EDTA抗凝血浆不可使用。
检测前所有试剂需平衡至室温
试剂盒组分：
• R-1缓冲液（铁浓度80μg/dL）：20mL ×1瓶（即用型）
• R-2螯合显色剂：3mL ×1瓶（即用型）
需用户自备：
• 蒸馏水
• 微量移液器及吸头
• 透明平底96孔板
• 酶标仪（主波长546nm）
[注意事项]：该不饱和铁结合力（UIBC）检测试剂盒(Bathophenanthroline)仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
